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Rice Functional Genomics and Agrobiotechnology
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The ChuLab mainly focuses on functional genomics and Agrobiotechnology by the
use of rice as a model system. As a major effort, we have generated approximately 25,000 independent T-DNA insertion lines in the rice japonica cv. Nipponbare background.
We are focusing on the screening for rice mutants involved in agronomically important traits, such as photosynthesis capacity, rice yield, and preharvest sprouting, characterizing of representative mutants to facilitate the applications in molecular breeding. The long-term goal is to use the knowledge, resources and tools obtained from our studies and combine approaches of genetics, molecular biology and biochemistry as well as the conventional marker-assistant selection method to improve complex agronomic traits in rice. We will be particularly interested in yield and preharvest sprouting, two key agronomic traits in rice production.
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Selected Publications
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1. Lin A^, Wang Y^, Tang J^, Xue P, Li C, Liu L, Hu B, Yang F, Loake GJ, Chu C
(2011) Nitric oxide and protein s-nitrosylation are integral to hydrogen peroxide induced leaf cell death in rice,
Plant Physiol. 158: 451-464
2. Wang H, Fang J, Liang C, He M, Li Q, Chu C (2011) Computation-assisted SiteFinding-PCR for isolating flanking sequence tags in rice,
BioTechniques 51: 421-423.
3.Sun C^, Liu L^, Tang J, Lin A, Zhang F, Fang J, Zhang G, Chu CC
(2011) RLIN1, encoding a putative coproporphyrinogen III oxidase, is involved in lesion initiation in rice,
J. Genet. Genomics.38(1):29-37.
4.Li C, Wang Y, Liu L, Hu Y, Zhang F, Sodmergen, Wang G, Schläppi MR,
Chu C (2011)
A rice plastidial nucleotide sugar epimerase is involved in galactolipid biosynthesis
and improves photosynthetic efficiency,
PLoS Genet. 7(7):e1002196.
5.Tang J, Zhu X, Wang Y, Liu L, Xu B, Li F, Fang J,
Chu C (2011) Semidominant mutations in the CC-NB-LRR-type
R gene, NLS1, lead to constitutive activation of defense responses in rice.
Plant J. 76: 145-156.
6.Hu B, Zhu C, Li F, Tang J, Wang Y, Lin A, Liu L, Che R,
Chu C (2011) LEAF TIP NECROSIS1 plays a pivotal role in regulation of multiple phosphate starvation responses in rice.
Plant Physiol. 156: 1101-1115.
7.Chai C^, Fang J^, Liu Y, Tong H, Gong Y, Wang Y, Liu M, Wang Y, Qian Q, Cheng Z, Chu C (2011) ZEBRA2, encoding a carotenoid isomerase, is involved in photoprotection in rice. Plant Mol. Biol. 75(3): 211-221
8.Li F^, Liu W^, Tang J,
Chen J, Tong H, Hu B, Li C, Fang J, Chen M, and Chu C (2010) Rice DENSE
AND ERECT PANICLE 2 is essential for determining panicle outgrowth and
elongation, Cell Res. 20: 838-849..
9.Ma Y, Liu L, Zhu C, Sun C, Xu B, Fang F, Tang J, Luo A,
Cao S, Li G, Qian Q, Xue Y,
Chu C
(2009) Molecular analysis of
rice plants harboring a
multi-functional T-DNA
tagging system, J.
Genet. Genomics
36(5): 267-276.
10.Guo X, Wu Y, Wang Y,
Chu CC
(2009) OsMSRA4.1 and
OsMSRB1.1, two rice
plastidial methionine
sulfoxide reductases, are
involved in abiotic stress
responses. Planta
230:227-238.
11.Tong H, Jin Y, Liu W, Li
F, Fang J, Yin Y, Qian Q,
Zhu L, Chu CC
(2009) DWARF AND LOW-TILLERING,
a new member of GRAS family,
plays positive roles in
brassinosteroid signaling in
rice. Plant J.
58: 803-816.
12.Yang Z, Wu Y, Li Y, Ling
HQ, Chu CC
(2009) OsMT1a, a type 1
metallothionein, plays the
pivotal role in zinc
homeostasis and drought
tolerance in rice,
Plant Mol. Biol.
70(1-2): 219-229.
13.Wang Q, Guan Y, Wu Y, Chen
H, Chen F, Chu C
(2008) Overexpression of a rice
OsDREB1F gene increases
salt, drought, and low
temperature tolerance in both
Arabidopsis and rice.
Plant Mol. Biol. 67:
589-602.
14.Bai X, Wang Q, Chu C
(2008) Excision of a selective
marker in transgenic rice using
a novel Cre/loxP system
controlled by a floral specific
promoter. Transgenic Res.
17:1035-1043.
15.Fang J, Chai C, Qian Q, Li
C, Tang J, Sun L, Huang Z,
Guo X, Sun C, Liu M, Zhang
Y, Lu Q, Wang Y, Lu C, Han
B, Chen F, Cheng Z, Chu C
(2008) Mutations
of genes in synthesis of the
carotenoid precursors of ABA
lead to preharvest sprouting and
photo-oxidation in rice.
Plant J. 54: 177-189.
16.Ding Y, Wang X, Su L, Zhai J, Cao S, Zhang D, Liu C, Bi Y, Qian Q, Cheng Z, Chu
C*, Cao X* (2006)
SDG714, a histone H3K9
methyltransferase, is involved in Tos17 DNA methylation and
transposition in rice.
Plant Cell. 19(1): 9-22 (*Corresponding author).
17.Liu X, Bai X, Wang X, Chu C
(2006) OsWRKY71, a rice transcription factor, is involved in rice
defense response. J. Plant Physiol. 164(8): 969-979.
18.Luo A, Qian Q, Ying H, Liu X,
Yin C, Lan Y, Tang J, Tang Z, Cao S, Wang X, Xia K,
Fu X, Luo D, Chu C (2006) EUI1, encoding a
putative cytochrome P450 monooxygenase, regulates the internodes
elongation by modulating GA responses in rice. Plant Cell
Physiol. 47(2):181-191.
19.Liu XQ, Bai XQ, Qian Q, Wang XJ, Chen MS, and Chu CC (2005) OsWRKY03, a rice
transcription activator that functions in defense signaling pathway
upstream of OsNPR1. Cell Res. 15(8): 593-603.
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